Remove Constraints of Process Development — MaXtar® S HC Pro Redefines Cation Resin Performance Boundaries

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With the continuous development of pharmaceutical industry and the continuous optimization of upstream processes, downstream purification is facing the pressure of increasing protein impurity content and sudden increase of protein expression. Based on this, BioLink launched the star product — MaXtar® S HC Pro Cation Exchange Chromatography Resin, this product plays an important role in antibody and recombinant protein projects due to its three characteristics of "broadness", "sharpness" and "stablility".

Excellent Salt Tolerance

MaXtar® S HC Pro is a strong cation exchange resin. As an improved version of MaXtar® S HC, it also features high capacity and high resolution, but MaXtar® S HC Pro has a wider working range and can provide customers with more process operation space.

DOE Experimental Design: Central Composite Experiment

Factor:

pH Range: 4.5/5.25/6.0
Cond Range: 0/50/100 mM NaCl

Response: 10% DBC binding capacity

Figure 1: MaXtar® S HC Pro 10% DBC Contour Map

Summary: DoE results show that MaXtar® S HC Pro resin still has a maximum of about 100 mg/mL 10% DBC under the loading condition containing 100 mM NaCl. When loading with saline solution, in order to obtain a higher capacity, the loading pH value can be appropriately reduced.

Excellent Impurity Removal Ability

MaXtar® S HC Pro resin, with a particle size of 50μm, is mostly used in polishing steps. It has good separation ability whether it is used in bind-elute or flow-through mode, and can effectively reduce low molecular impurities and high molecular impurities from products.

Test Conditions:

Sample: III Collagen
Column: 4.7 mL prepacked column, column height 10 cm
Equilibration Buffer: 80 mM HAc, pH 2.8
Regeneration Buffer: 20 mM PB, 1 M NaCl, pH 8.0

figure-2-maxtar-s-hc-pro-flow-through-mode-chromatogram.jpg

Figure 2: MaXtar® S HC Pro flow-through mode chromatogram

Samples Name	SEC Purity
Samples Name	HMW (%)	Monomer (%)	LMW (%)
Load	1.06	79.317	19.623
Elution	0.51	99.39	0.10

Table 1: SEC-HPLC (%) Test Results

Summary: The results show that when MaXtar® S HC Pro uses flow-through mode under the condition of 90g/L of flow-through protein, the purity of flow-through solution reaches 99.39%, and the initial low molecular impurities decreases from 19.623% to 0.1%, and almost all of them are removed. This case shows MaXtar® S HC Pro's excellent impurity removal capability.

Robustness for Industrial Scale-up

MaXtar® S HC Pro’s matrix is MaXtar, an improved agarose-based matrix, which has the characteristics of high rigidity and low back pressure, and has larger scale-up space in industrial scale-up. MaXtar® S HC Pro has been verified the robustness of scaling up in practice.

Pressure-flow rate curve (column diameter: 300 mm):

Figure 3: MaXtar® S HC Pro Pressure-Flow Rate Curve

Case:

Column Volume: 40 L
Column Height: 14.1 cm
Column Diameter: 600 mm

Figure 4: Process flow pressure performance

Summary: The figure above shows that in the whole process of industrial production, the whole process pressure of MaXtar® S HC Pro is ≤ 1. 2 bar, indicating that the back pressure of the resin is small and it has robust scale-up performance.

In short, MaXtar® S HC Pro's excellent salt tolerance, high resolution and outstanding scalability can provide more choices for process development. BioLink also hopes that MaXtar® S HC Pro chromatography resin can help more customers achieve their ideal purification process goals.

Product Catalog Number

Products Name	Cat. No.	Pack Size
MaXtar^® S HC Pro	1012-1841	25 mL
	1012-1842	100 mL
	1012-1843	500 mL
	1012-1844	1 mL
	1012-1835	5 mL
	1012-1836	10 mL
	1012-1837	20 mL
Chrom-Trap^®MaXtar^®S HC Pro 1mL	2012-1841	1×1mL
Chrom-Trap^®MaXtar^® S HC Pro 4.7mL	2012-1842	1×4.7mL
Chrom-Trap^®MaXtar^®S HC Pro 5mL	2012-1843	1×5mL

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