Purification Techniques | Boost Antibody Purification ——BioLink MaXtar® Protein L Application Cases

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In the R&D and production process of antibody drugs, an efficient and stable purification process is a key link to ensure product quality and production efficiency. Since BioLink antibody affinity resin MaXtar^® Protein L launches, Protein L has become an important tool for the purification of various antibody fragments (such as Fab, scFv, Dab, etc.) and intact antibodies (such as IgG, IgM and bispecific antibodies) by virtue of its specific binding to kappa light chain variable region.

figure-1-structural-form-of-protein-l-binding-to-molecules.jpg

Figure 1: Structural form of Protein L binding to molecules

Core Competencies of MaXtar^® Protein L Resin

Highly cross-linked agarose-based matrix for improved process efficiency.
Stronger alkali resistance, low ligand shedding, prolonged service life.
Stable batch-to-batch consistency.
Protein L ligand protected by intellectual property rights, perfect regulatory support files (RSF).
200 L single batch production capacity up to 200 L, short delivery time, stable supply chain, and high cost performance.

Product Parameters

Manufacturer	BioLink
Resin	MaXtar® Protein L
Matrix	Highly Cross-Linked Agarose
Ligand	rProtein L
Average Particle Size	65μm
Dynamic Binding Capacity	≥50 mg IgG1/mL(RT 6min)
Maximum Pressure Resistance	0.5 MPa
CIP	0.05~0.1M NaoH
pH Stability	3~12 (Long-Term) 2~13 (Short-Term)
Storage Conditions	20% Ethanol, 2~8℃
*Binding capacity depends on the specificity of the antibody molecule

Table 1: MaXtar^®Protein L product parameters

Stable Batch-to-batch Consistency

Figure 2: The dynamic binding capacity RSD of 3 batches is < 5%, and the consistency between batches is relatively stable.

Application Case Sharing —— Fab Purification

Column: Chrom-LinX^® 16 mm * 18 cm
CV: 36 mL

Step	Buffer	CV	Residence Time (min)
Sanitization	0.05 mol/L NaOH	3.0	5
Equilibration	25 mM Tris,0.15 M NaCl,pH 7.4	3.0	5
Sample Loading	Fab Cell Supernatant	Loading at 27 mg/mL	5
Re-equilibration	25 mM Tris,0.15 M NaCl,pH 7.4	5.0	5
Wash I	50 mM NaAC-HAc,1M NaCl,pH 5.5	3.0	5
Wash II	50 mM NaAC-HAc,pH 5.5	3.0	5
Elution	50 mM NaAC-HAc,pH 3.0	5.0	5
Post-sanitization	0.05mol/L NaOH	3.0	5
Storage	20% Ethanol	2.0	5

Table 2: Process Conditions

Figure 3: MaXtar^® Protein L purified Fab fragments

CAT. NO.

Resin	Yield A(%)	SEC(%)	Collected CV
MaXtar® Protein L	88	99	1.5

Table 3: MaXtar^® Protein L purification data

Summary: After purification of MaXtar^® Protein L, the purity and recovery performance are in line with expectations, and it still has good elution performance under sodium acetate system.

References

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23 Sep 2023

Purification Techniques | Boost Antibody Purification ——BioLink MaXtar® Protein L Application Cases

Core Competencies of MaXtar® Protein L Resin

Product Parameters

Stable Batch-to-batch Consistency

Application Case Sharing —— Fab Purification

Core Competencies of MaXtar^® Protein L Resin