Purification Guide: Hydration Products Solution

In the field of life science research, the stability of biomolecules such as proteins is inseparable from the precise control of pH by buffer salts. Different types of buffers have become one of the most commonly used materials in the field of biological sciences.

However, the process of laboratory-made buffer is often full of "hidden costs"—from calculating the dosage of reagents, accurately weighing materials, to adjusting the pH, pipetting and making up the volume, every step takes a lot of time; If scenarios such as cell culture and medical research are involved, it is necessary to ensure that the solution is sterile and endotoxin-free, and the operational complexity is further upgraded.

Laboratory-made Buffer

Pain Points Hidden Burried in Steps

Even if the standard recipe can be queried from the Internet, the laboratory-made buffer still needs to overcome multiple difficulties, including:

• Weighing Accuracy Difficulties: The concentration of buffer salt is mostly mmol/L. When preparing small volumes, it is necessary to accurately weigh it with a thousand or even ten thousand balance, and the amount of buffer salt crystal water must be carefully checked. Once the difference of crystal water is ignored, it may directly lead to buffer concentration deviation and experimental failure;

  
  

• Pollution Risk Prevention and Control: The dissolution process needs to use purified water or ultrapure water to avoid the precipitation of Ca²⁺, Mg²⁺ plasma and phosphate, and prevent the introduction of microorganisms and endotoxins; If aseptic treatment is required, sterilization and filtration must be completed in an ultra-clean table (no humid heat sterilization, otherwise it will lead to hydrolysis of buffered saline, volatilization of components, and pH fluctuation);

  
  

• Time-consuming and Labor-intensive Bottlenecks: The pH adjustment process often requires repeated testing, slow dropwise addition of acid-base reagents, and filtering and removing particles after making up volume. The whole process is cumbersome, especially for experiments with strict requirements for endotoxin. The container needs to be soaked in acid-base or dry heat treated at high temperature in advance, which further takes up scientific research time.

  
  

To solve these pain points, various dry powder buffers have been introduced on the market, which only need to be dissolved and filtered before they can be used. However, such buffers generally cannot guarantee sterility, and their dissolution process is usually carried out in an unclean environment. Even if they are used in protein purification processes that require non-sterility requirements, microorganisms are easy to breed with the increase of storage time. The prefabricated sterile and endotoxin-free buffer solution (i.e. "hydration product") has become the preferred solution in the pharmaceutical and scientific research fields by virtue of its advantages of "ready-to-use and stable quality"-it can not only eliminate the cumbersome preparation process, but also It can ensure sterile and endotoxin-free properties through strict quality control, and sealed packaging supports long-term storage, completely releasing scientific research manpower.

BioLink Hydration Products

Lab-grade Quality Makes Experiments More "Worry-free"

BioLink is deeply involved in scientific research needs and has launched two core hydration products, water for injection (WFI) and ready-to-use buffer. With high standard quality and flexible service, it has become the trusted choice of scientific researchers.

Water for Injection: The "cornerstone" of experiment, pure and worry-free

BioLink water for injection strictly follows authoritative standards such as the Chinese Pharmacopoeia 2025 and the United States Pharmacopoeia to ensure the purity and safety of water quality from the source.

• Strict Quality Control: Ensure that key indicators such as sterility and endotoxin-free meet the standards, and avoid impurities affecting the experimental results;

• Multiple Sizes Available: Provide packaging of different volumes such as 0.5 L, 1 L, and 2 L to meet diverse needs from laboratory experiments to pilot scales;

• Efficiency Improvement: It is directly used to clean experimental equipment and prepare buffer solution, saving the time and labor cost of self-made purified water, and allowing researchers to focus on core experimental links.

• Applicable Scenarios: Various life science experiments such as cell culture, genetic engineering, and drug research and development are especially suitable for precision operations with stringent water quality requirements.

Figure 1. HydroLinX^® Water for Injection in bottles

Ready-to-Use Buffer

Accurate and Stable, Customized to Meet Needs

BioLink's ready-to-use buffers adopt a dual-track mode of "standardization + customization", taking into account experimental reliability and flexibility:

• Strict Quality Control, Safety and Controllable: Each batch has passed four core tests: biocompatibility verification, subvisible particle test, bacterial endotoxin test, and validity period verification to ensure that the purity, pH value, and concentration of the solution are stable and reliable, which fully meets the safety and safety requirements of scientific research experiments. High requirements for effectiveness;

• Customized Services to Suit Special Needs: The buffer type, concentration, and pH value can be customized according to the experimental scenario, and even special quality inspections such as RNase-free and DNase-free can be carried out to solve the "adaptation problem" of special experiments;

• Ready to Use, Double the Efficiency: There is no need for cumbersome preparation and adjuastment, and it can be directly used in experiments after opening, greatly reducing manpower investment, avoiding batch differences caused by manual operations and improving experimental repeatability.

  
  

Figure 2: HydroLinX^® Buffers

Main Product Order Information

Table 1: Water for injections in bottles

Table 2: Buffers in bottles

Conclusion

From basic hydration to customized buffers, BioLink hydration products take "high standards, high efficiency, and high adaptability" as the core to build a stable and reliable "material cornerstone" for scientific research experiments. Choose BioLink, bid farewell to cumbersome operations, and let scientific research focus on innovative breakthroughs!