ChromaX® MonoPM™ 65S: An“All-Round Performer” in Cation Exchange Chromatography

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Pigments hard to remove? Peak tailing?

Limited flow rate? Insufficient capacity?

Your ion exchange chromatography may simply be missing the right resin!

Cation exchange chromatography is a core technology for purifying biomolecules based on charge differences. Negatively charged functional groups on the resin selectively bind positively charged target molecules, which are then gently eluted under specific conditions—delivering both efficiency and mild processing. As a result, it is widely used in the separation and purification of various biomolecules, including monoclonal antibodies, recombinant proteins, antibody fragments, and vaccines.

A Powerful New Release

BioLink proudly introduces the new ChromaX^® MonoPM™ 65S chromatography resin. Built on monodisperse 65 μm rigid polymer microspheres, it delivers sharp elution peaks even at high dynamic binding capacities, while tolerating higher pressure and flow rates, significantly improving purification efficiency. Whether for early-stage process development or pilot-scale production, it consistently provides high-purity and high-recovery results. More than just a purification tool, it is a reliable platform from basic research to scale-up manufacturing.

Choose ChromaX^®MonoPM™ 65S to make every purification step cleaner, faster, and more efficient!

Figure 1: ChromaX^® MonoPM™ 65S

Technical Parameters

Name	Description
Chromatography Resin Type	Cation Exchange Chromatography Resin
Functional Group	-SO₃H
Matrix	Polymethacrylate
Mean Particle Size	65 μm
Maximum Flow Rate	700 cm/h
Pressure Resistance	≤ 1 MPa (10 bar)
Operating Temperature	4–30°C
Storage Conditions	4–30°C, 20% Ethanol

Table 1: Basic Parameters of ChromaX^® MonoPM™ 65S Resin

Core Advantages

High Resolution: Sharp peak shapes enable more thorough impurity removal.
Ultra-High Capacity: A small amount of resin can capture a large quantity of target protein, significantly reducing experimental costs.
Excellent Flow Rate–Pressure Performance: Supports operation at higher flow rates, greatly shortening purification time.
Outstanding Stability: Strict quality control ensures batch-to-batch consistency and consistently reliable results.

Let Data Lead the Way: Directly to the Optimal Solution

01 Excellent Batch-to-Batch Consistency

Using identical conditions, different batches of the resin were tested for ligand density and dynamic binding capacity. The data, presented as the mean values and standard deviations of three consecutive batches, demonstrate excellent batch-to-batch consistency.

Figure 1: Ligand Density and Binding Capacity Data

02 Linear Pressure–Flow Relationship Across the Entire Range with No Inflection or Collapse

Column packing conditions for this experiment: ID 16 mm, height approximately 20 cm, compression factor 1.03. The pressure–flow curves were tested using 0.1 M NaCl and purified water. Data are presented as the mean values and standard deviations of three consecutive batches. The results show a fully linear pressure–flow relationship across the entire range, with no inflection points or bed collapse.

figure-2-pressure-flow-curve-at-0.1-m-nacl.jpg

Figure 2: Pressure–Flow Curve at 0.1 M NaCl

Figure 3: Pressure–Flow Curve in H₂O

03 Application Case: Purification of Recombinant Human Serum Albumin

Resin: ChromaX^®MonoPM™ 65S chromatography resin
Column: 4.7 mL prepacked column
Sample: Recombinant human serum albumin
Equilibration Buffer: 100 mM NaAc, 5 mM sodium caprylate, pH 4.7
Elution Buffer: 500 mM NaAc, 5 mM sodium caprylate, pH 4.7
Regeneration Buffer: 20 mM HAc, 10 mM NaH₂PO₄, 1 M NaCl, pH 4.7
Cleaning Solution: 0.5 M NaOH
Flow Rate: 100 cm/h

Figure 4: Experimental Chromatogram

Figure 5: SDS-PAGE Results

Figure 6: Pigment Removal Performance

Product Information

Product Name	Catalog No.	Pack Size
ChromaX^®MonoPM™ 65S	1212-1567	20L
	1212-1566	10L
	1212-1565	5L
	1212-1564	1L
	1212-1563	500mL
	1212-1562	100mL
	1212-1561	25mL

Table 2: Product Information for ChromaX^® MonoPM™ 65S

References

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12 May 2026