Downstream bioprocessing refers to the later stages of the bioprocessing production process, which typically involve purifying and refining the bioprocessing product. Protein chromatography is a commonly used technique in downstream bioprocessing for purifying and refining proteins.
Protein Chromatography Working Principle
Protein chromatography works by selectively binding proteins to a stationary phase, typically a resin, based on specific interactions between the protein and the resin. The proteins can then be eluted from the resin, resulting in a purified and concentrated sample.
The use of protein chromatography in downstream bioprocessing offers several benefits, including:
Improved product purity: Protein chromatography can remove impurities and contaminants, resulting in a highly purified protein product.
Increased yield: Protein chromatography can increase the yield of the bioprocessing product, as it enables the selective capture and purification of the target protein.
Increased efficiency: The use of protein chromatography can streamline the downstream bioprocessing process, reducing the time and cost associated with purifying and refining the bioprocessing product.