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CytoLinX® GB 1-20 L Benchtop Glass Bioreactors

The CytoLinX® GB benchtop glass bioreactors are available in 1 L, 3L, 7L, 15L and 20 L with a new design and high flexibility, allowing the use of existing brands of electrodes, tubes, gas distributors and strring paddle. The bioreactor is composed of a glass vessel and a control cabinet; it is widely used in the upstream process development of biologics, suitable for exploration of high-throughput parameter conditions and optimization of the culture process, and applicable for the culture of various cells, including suspension cells, adherent cells, plant cells, and insect cells.

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Features of CytoLinX® GB 1-20 L Benchtop Glass Bioreactors

Features of CytoLinX® GB 1-20 L Benchtop Glass Bioreactors


CytoLinX® GB Control Cabinet

The CLX-GB control cabinet includes control hardware and underlying hardware modules. The control hardware is built with Siemens win CC framework, which meets requirements from development to manufacturing and complies with 21 CFR Part 1.


The underlying hardware modules are all built with imported brands, featuring high stability. In addition, a variety of PAT tools can be flexibly configured to meet different customer needs and application scenarios.


ACLX-GB control cabinet can be interfacing with different models of glass vessels, achieving versatile use with a single unit.


The CLX-GB vessel adopts a top stirring mode and allows a flexible combination of microbubble and large bubble, promoting the optimization of the culture process, further improving cell density as well as maintaining cell viability.

CytoLinX® GB main control interface

Figure 2. CytoLinX® GB main control interface


Applications of CytoLinX® GB 1-20 L Benchtop Glass Bioreactors

CytoLinX® GB for ADC Conjugation Process Optimization

BioLink provides an automated platform for the optimization of the ADC conjugation process. Features such as precise temperature control and programmable automatic dosing ensure high repeatability and stability of the reaction system, and the kettle design is more feasible for manufacturing and linear scale-up. The synthesis platform has excellent scalability and can be equipped with online pH sensors to enhance process development monitoring and control. In addition, the automatic program is designed to significantly avoid errors caused by manual operations, thereby ensuring consistency between synthesis batches.


CytoLinX® GB for mRNA Production

The development difficulty and the key technical points of mRNA technology lie in the synthetic modification and delivery systems. In Vitro Transcription (IVT) is the most efficient method for batch synthesis of mRNA. It is a relatively complex enzymatic process that requires the addition of enzymes, nucleotides, and other related reagents, in addition to the DNA template. BioLink provides a fully automated IVT synthesis platform that enables efficient linear scale-up from lab-scale development to scale up production. With features such as precise temperature control and programmable automatic dosing, the CytoLinX® GB benchtop glass bioreactors ensure the high repeatability and stability of the synthesis system, and the automatic program design significantly avoids the errors caused by manual operations to ensure the consistency between synthesis batches.


Configuration of CytoLinX® GB 1-20 L Benchtop Glass Bioreactors


Vessel (single-wall)

Total volume

1 L

3 L

7 L

15 L

20 L

Max. working volume

0.8 L

2.4 L

5.6 L

12 L

16 L

Min. working volume

0.2 L

0.6 L

1.4 L

3 L

8 L

Working 

height to 

diameter ratio (H/D)

1.5/1

1.5/1

1.8/1

1.45/1

1.5/1

Material



High silicon borate



Vessel

(jacket)

Max. working volume

NA

2.4 L

5.6 L

12 L

NA

Min. working volume

NA

0.6 L

1.4 L

3 L

NA

Working diameter to height ratio (H/D)

NA

1.5/1

1.8/1

1.4/1

NA

Jacket volume

NA

1.2 L

2.4 L

7.4 L

NA

Material



High silicon borate



Vessel

cover


Port

M30*1 M18*1.5*3

10 mm*6

6 mm*3

M30*1 M18*1.5*6

12 mm*2

10 mm*9

M30*1 M18*1.5*6

12 mm*2

10 mm*10

69 mm*1

27 mm*6

12 mm*2

10 mm*10

69 mm*1

27 mm*6

12 mm*2

10 mm*10

Material

SS316L

Stirring

system

Mode


Top servo brushless motor

Stirring speed

0-1500 rpm

Sensor

pH

Hamilton smart pH electrode, VP8 joint

DO

Hamilton smart optical dissolved oxygen electrode, VP8 joint

Temperature

Pt 100, Grade A, 0-150°C

Level/antifoam

Resistance type, automatic level control for adding antifoam (optional)

Others

pCO2 electrode (optional), cell density electrode (optional), conductance (optional), Raman electrode (optional), etc.




Gas control

Ventilation mode

Surface ventilation/deep ventilation

Gas type

Air, O2, CO2, N2

Control method

0-6 channel mass flow meter (MFC) /0-4 channel rotameter

Gas distributor

L-shaped bubble/annular bubble/microbubble (5-200 um)

Tail gas

Stainless steel condensing reflow (optional)

Peristaltic

pump

Peristaltic pump

2-4 variable speed pumps

Speed

0.1-300 rpm

Reserve external pump port

0-2 variable speed pumps (optional)

Temperature

Control method

Electric heating blanket (Single-wall vessel)/TCU heating (jacketed vessel)

Weighing

Reserve external weight port

0-4 variable speed weights (optional)

Control

system

Controller

Industrial PLC

Interface

23" PC

Compliance

Conforming to 21 CFR PART 11

Integrity test

Integrity tester

Detection range: 0-350 mbar (optional)

Ordering Information

1 L benchtop glass bioreactor

1 L benchtop glass bioreactor

Cat No.

Specification

Glass reactor



1 L glass reactor, single-wall (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB01LGS-001

1 L

1 L glass reactor, jacket (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB01LGJ-001

1 L

1 L single-wall glass reactor

CGB01LVS-001

1 L

1 L jacketed glass reactor

CGB01LVJ-001

1 L

1 L single-wall, jacketed universal stainless steel roof

CGB01LSR-001

1 L

1 L single-wall stainless steel bracket

CGB01LSR-002

1 L

1 L jacketed stainless steel bracket

CGB01LSR-015

1 L

Stir



1 L mixing assembly

CGB01LSR-003

OD: M30

1 L marine mixing paddle

CGB01LSR-004

OD: 38 mm

1 L flat blade turbine mixing paddle

CGB01LSR-005

OD: 38 mm

1 L baffle

CGB01LSR-006

OD: 6 mm

Gas



1 L bottom intake tube (large bubble)

CGB01LSR-007

OD: 10 mm

1 L annular bottom intake tube (large bubble)

CGB01LSR-013

OD: 10 mm

1-20 L surface ventilation assembly

CGB01-20LSR-001

OD: 10 mm

Sensors and related accessories



1 L Hamilton ARC pH sensor

CGB01LER-001

160 mm long

1 L Hamilton ARC DO sensor

CGB01LER-002

160 mm long

1 L Pt100 temperature sensor

CGB01LER-003

1 L, 160 mm long

1 L liquid level/foam sensor

CGB01LER-004

OD: 10 mm

1 L stainless steel temperature casing

CGB01LSR-009

OD: 10 mm

1-7 L electrode adapter

CGB01-07LSR-002

OD: M18*1.5

Tail gas



1-7 L condensation reflux

CGB01-07LSR-001

OD: M18*1.5


1 L benchtop glass bioreactor

Cat No.

Specification

Cooler



1 L heat exchange tube

CGB01LSR-014

OD: 10 mm

Feed and sampling line



1-7 L three-inlet components

CGB01-07LSR-004

OD: M18*1.5

1-20 L 10-mm feed tube

CGB01-20LSR-002

OD: 10 mm

1-20 L 12-mm feed tube

CGB01-20LSR-003

OD: 12 mm

1 L harvest tube

CGB01LSR-010

OD: 10 mm

1 L sampling tube

CGB01LSR-011

OD: 10 mm

Plug



1-7 L M18*1.5 plug

CGB01-07LSR-003

OD: M18*1.5

1-20 L 10-mm plug

CGB01-20LSR-004

OD: 10 mm

1-20 L 12-mm plug

CGB01-20LSR-005

OD: 12 mm

1 L 6-mm plug

CGB01LSR-012

OD: 6 mm

1-20 L electrode plug

CGB01-20LSR-006

Thread: PG13.5

Heated blanket



1 L heated blanket

CGB01LER-005

1 L: 360*100, 230 V, 80 W


3 L benchtop bioreactor

3 L benchtop bioreactor

Cat No.

Specification

Glass reactor



3 L glass reactor, single-wall (bracket, glass reaction vessel, stainless steel roof and bracket)


CGB03LGS-001


3 L

3 L glass reactor, jacket (bracket, glass reaction vessel, stainless steel roof and bracket)


CGB03LGJ-001


3 L

3 L single-wall glass reactor

CGB03LVS-001

3 L

3 L jacketed glass reactor

CGB03LVJ-001

3 L

3 L single-wall, jacketed universal stainless steel roof

CGB03LSR-001

3 L

3 L single-wall stainless steel bracket

CGB03LSR-002

3 L

3 L jacketed stainless steel bracket

CGB03LSR-004

3 L

Stir



3 L mixing assembly

CGB03LSR-005

OD: M30

3 L marine mixing paddle

CGB03LSR-006

OD: 45 mm

3 L flat blade turbine mixing paddle

CGB03LSR-007

OD: 45 mm

3 L baffle

CGB03LSR-008

OD: 10 mm

Gas



3 L bottom intake tube (large bubble)

CGB03LSR-009

OD: 10 mm

3 L bottom intake tube (microbubble)

CGB03LSR-010

OD: 10 mm

3 L annular bottom intake tube (large bubble)

CGB03LSR-015

OD: 10 mm

1-20 L surface ventilation assembly

CGB01-20LSR-001

OD: 10 mm

1-7 L microbubble tip, 20 um

CGB01-07LSR-006


1-7 L microbubble tip, other pore size

Customized


Sensors and related accessories



3 L Hamilton ARC pH sensor

CGB03LER-001

225 mm long

3 L Hamilton ARC DO sensor

CGB03LER-002

225 mm long

3 L Pt100 temperature sensor

CGB03LER-003

3 L, 200 mm long

3 L liquid level/foam sensor

CGB03LER-004

OD: 10 mm

1-7 L electrode adapter

CGB01-07LSR-002

OD: M18*1.5

3 L stainless steel temperature casing

CGB03LSR-011

OD: 10 mm


3 L benchtop bioreactor

Cat No.

Specification

Tail gas



1-7 L condensation reflux

CGB01-07LSR-001

OD: M18*1.5

Cooler



3 L heat exchange tube

CGB03LSR-012

OD: 10 mm

Feed and sampling line



1-7 L three-inlet components

CGB01-07LSR-004

OD: M18*1.5

1-20 L 10-mm feed tube

CGB01-20LSR-002

OD: 10 mm

1-20 L 12-mm feed tube

CGB01-20LSR-003

OD: 12 mm

3 L harvest tube

CGB03LSR-013

OD: 10 mm

3 L sampling tube

CGB03LSR-014

OD: 10 mm

Plug



1-7 L M18*1.5 plug

CGB01-07LSR-003

OD: M18*1.5

1-20 L 10-mm plug

CGB01-20LSR-004

OD: 10 mm

1-20 L 12-mm plug

CGB01-20LSR-005

OD: 12 mm

1-20 L electrode plug

CGB01-20LSR-006

Thread: PG13.5

Heated blanket



3 L heated blanket

CGB03LER-005

3 L: 485*180, 230 V, 100 W


7 L benchtop bioreactor

7 L benchtop bioreactor

Cat No.

Specification

Glass reactor



7 L glass reactor, single-wall (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB07LGS-001

7 L

7 L glass reactor, jacket (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB07LGJ-001

7 L

7 L single-wall glass reactor

CGB07LVS-001

7 L

7 L jacketed glass reactor

CGB07LVJ-001

7 L

7 L single-wall, jacketed universal stainless steel roof

CGB07LSR-001

7 L

7 L single-wall stainless steel bracket

CGB07LSR-002

7 L

7 L jacketed stainless steel bracket

CGB07LSR-004

7 L

Stir



7 L mixing assembly

CGB07LSR-005

OD: M30

7 L marine mixing paddle

CGB07LSR-006

OD: 60 mm

7 L flat blade turbine mixing paddle

CGB07LSR-007

OD: 60 mm

7 L baffle

CGB07LSR-008

OD: 10 mm

Gas



7 L bottom intake tube (large bubble)

CGB07LSR-009

OD: 10 mm

7 L bottom intake tube (microbubble)

CGB07LSR-010

OD: 10 mm

7 L annular bottom intake tube (large bubble)

CGB07LSR-015

OD: 10 mm

1-20 L surface ventilation assembly

CGB01-20LSR-001

OD: 10 mm

1-7 L microbubble tip, 20 um

CGB01-07LSR-006


1-7 L microbubble tip, other pore size

Customized


Sensors and related accessories



7 L Hamilton ARC pH sensor

CGB07LER-001

325 mm long

7 L Hamilton ARC DO sensor

CGB07LER-002

325 mm long

7 L Pt100 temperature sensor

CGB07LER-003

7 L, 300 mm long

7 L liquid level/foam sensor

CGB07LER-004

OD: 10 mm

1-7 L electrode adapter

CGB01-07LSR-002

OD: M18*1.5

7 L stainless steel temperature casing

CGB07LSR-011

OD: 10 mm


7 L benchtop bioreactor

Cat No.

Specification

Tail gas



1-7 L condensation reflux

CGB01-07LSR-001

OD: M18*1.5

Cooler



7 L heat exchange tube

CGB07LSR-012

OD: 10 mm

Feed and sampling line



1-7 L three-inlet components

CGB01-07LSR-004

OD: M18*1.5

1-20 L 10-mm feed tube

CGB01-20LSR-002

OD: 10 mm

1-20 L 12-mm feed tube

CGB01-20LSR-003

OD: 12 mm

7 L harvest tube

CGB07LSR-013

OD: 10 mm

7 L sampling tube

CGB07LSR-014

OD: 10 mm

Plug



1-7 L M18*1.5 plug

CGB01-07LSR-003

OD: M18*1.5

1-20 L 10-mm plug

CGB01-20LSR-004

OD: 10 mm

1-20 L 12-mm plug

CGB01-20LSR-005

OD: 12 mm

1-20 L electrode plug

CGB01-20LSR-006

Thread: PG13.5

Heated blanket



7 L heated blanket

CGB07LER-005

7 L: 575*165, 230 V, 178 W


15 L benchtop bioreactor

15 L benchtop bioreactor

Cat No.

Specification

Glass reactor



15 L glass reactor, single-wall (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB15LGS-001

15 L

15 L glass reactor, jacket (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB15LGJ-001

15 L

15 L single-wall glass reactor

CGB15LVS-001

15 L

15 L jacketed glass reactor

CGB15LVJ-001

15 L

15 L&20 L single-wall, jacketed universal stainless steel roofs

CGB15&20LSR-008

15 L

15 L single-wall stainless steel bracket

CGB15LSR-002

15 L

15 L jacketed stainless steel bracket

CGB15LSR-004

15 L

Stir



15 L mixing assembly

CGB15LSR-005

OD: 69 mm

15&20 L marine mixing paddles

CGB15&20LSR-004

OD: 74 mm

15&20 L flat blade turbine mixing paddle

CGB15&20LSR-005

OD: 75 mm

15 L baffle

CGB15LSR-006

OD: 10 mm

Gas



15 L bottom intake tube (large bubble)

CGB15LSR-007

OD: 10 mm

15 L bottom intake tube (microbubble)

CGB15LSR-008

OD: 10 mm

15 L annular bottom intake tube (large bubble)

CGB15LSR-013

OD: 10 mm

1-20 L surface ventilation assembly

CGB01-20LSR-001

OD: 10 mm

15&20 L microbubble tips, 20 um

CGB15&20LSR-009


15&20 L microbubble tips, other pore size

Customized


Sensors and related accessories



15&20 L Hamilton ARC pH sensors

CGB15&20LER-001

425 mm long

15&20 L Hamilton ARC DO sensors

CGB15&20LER-002

425 mm long

15&20 L Pt100 temperature sensors

CGB15&20LER-003

15 L&20 L, 400 mm long

15&20 L liquid level/foam sensors

CGB15&20LER-004

OD: 10 mm

15&20 L electrode adapters

CGB15&20LSR-002

OD: 27 mm

15 L stainless steel temperature casing

CGB15LSR-009

OD: 10 mm


15 L benchtop bioreactor

Cat No.

Specification

Tail gas



15&20 L condensation reflux

CGB15&20LSR-001

OD: 27 mm

Cooler



15 L heat exchange tube

CGB15LSR-010

OD: 10 mm

Feed and sampling line



15&20 L three-inlet components

CGB15&20LSR-003

OD: 27 mm

1-20 L 10-mm feed tube

CGB01-20LSR-002

OD: 10 mm

1-20 L 12-mm feed tube

CGB01-20LSR-003

OD: 12 mm

15 L harvest tube

CGB15LSR-011

OD: 10 mm

15 L sampling tube

CGB15LSR-012

OD: 10 mm

Plug



15 L&20 L 27-mm plug

CGB15&20LSR-006

OD: 27 mm

1-20 L 10-mm plug

CGB01-20LSR-004

OD: 10 mm

1-20 L 12-mm plug

CGB01-20LSR-005

OD: 12 mm

1-20 L electrode plug

CGB01-20LSR-006

Thread: PG13.5

Heated blanket



15 L&20 L heated blankets

CGB020LER-005

15 L&20 L: 760*200, 230 V,

383 W


20 L benchtop bioreactor

20 L benchtop bioreactor

Cat No.

Specification

Glass reactor



20 L glass reactor, single-wall (bracket, glass reaction vessel, stainless steel roof and bracket)

CGB20LGS-001

20 L

20 L single-wall glass reactor

CGB20LVJ-001

20 L

15 L&20 L single-wall, jacketed universal stainless steel roofs

CGB15&20LSR-008

20 L

20 L single-wall stainless steel bracket

CGB20LSR-002

20 L

Stir



20 L mixing assembly

CGB20LSR-003

OD: 69 mm

15&20 L marine mixing paddles

CGB15&20LSR-004

OD: 74 mm

15&20 L flat blade turbine mixing paddle

CGB15&20LSR-005

OD: 75 mm

20 L baffle

CGB20LSR-004

OD: 10 mm

Gas



20 L bottom intake tube (large bubble)

CGB20LSR-005

OD: 10 mm

20 L bottom intake tube (microbubble)

CGB20LSR-006

OD: 10 mm

20 L annular bottom intake tube (large bubble)

CGB20LSR-011

OD: 10 mm

1-20 L surface ventilation assembly

CGB01-20LSR-001

OD: 10 mm

15&20 L microbubble tips, 20 um

CGB15&20LSR-009


15&20 L microbubble tips, other pore size

Customized


Sensors and related accessories



15&20 L Hamilton ARC pH sensors

CGB15&20LER-001

425 mm long

15&20 L Hamilton ARC DO sensors

CGB15&20LER-002

425 mm long

15&20 L Pt100 temperature sensors

CGB15&20LER-003

15 L&20 L, 400 mm long

15&20 L liquid level/foam sensors

CGB15&20LER-004

OD: 10 mm

15&20 L electrode adapters

CGB15&20LSR-002

OD: 27 mm

20 L stainless steel temperature casing

CGB20LSR-007

OD: 10 mm

Tail gas



15&20 L condensation reflux

CGB15&20LSR-001

OD: 27 mm

Cooler



20 L heat exchange tube

CGB20LSR-008

OD: 10 mm


20 L benchtop bioreactor

Cat No.

Specification

Feed and sampling line



15&20 L three-inlet components

CGB15&20LSR-003

OD: 27 mm

1-20 L 10-mm feed tube

CGB01-20LSR-002

OD: 10 mm

1-20 L 12-mm feed tube

CGB01-20LSR-003

OD: 12 mm

20 L harvest tube

CGB20LSR-009

OD: 10 mm

20 L sampling tube

CGB20LSR-010

OD: 10 mm

Plug



15&20 L 27-mm plug

CGB15&20LSR-006

OD: 27 mm

1-20 L 10-mm plug

CGB01-20LSR-004

OD: 10 mm

1-20 L 12-mm plug

CGB01-20LSR-005

OD: 12 mm

1-20 L electrode plug

CGB01-20LSR-006

Thread: PG13.5

Heated blanket



15&20 L heated blankets

CGB15&20LER-005

15 L&20 L: 760*200, 230 V,

383 W


Supporting device

Cat No.

Specification

Integrity tester

CGB01-20LIT-001

Suitable for 1-20 L vessels

TCU

CGB01-20LTC-001

Suitable for 1-15 L jacketed vessel and benchtop mixing



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