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immobilized metal affinity chromatography
immobilized metal affinity chromatography

Ni Chromstar Affinity Chromatography Resins

Ni Chromstar FF/HP is an affinity chromatography resin made by pre-chelating transition metal ion Ni2+ to the Chromstar matrix with NTA as its ligand. The chromatography resin utilizes the interactions between Ni2+ and side chains of some amino acids (mainly histidine) in the protein to separate and purify proteins with and without these amino acids and with high or low amounts of these amino acids. Ni Chromstar FF/HP is the preferred choice for purification of His-tagged proteins for its high load, easy regeneration and low cost.

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Features of Ni Chromstar Affinity Chromatography Resins

Histidine-tagged proteins purification:


Specifications of Ni Chromstar Affinity Chromatography Resins

ProductDynamic binding capacityMean
particle size
(μm)
Max. back
pressure
(MPa)
Max. flow
velocity
(cm/h)
QuantityProduct code

Ni Chromstar FFspacer.gif

40 mg His-tagged protein/mL900.340025 mL1021-1131
100 mL1021-1132
500 mL1021-1133
1 L1021-1134
5 L1021-1135
10 L1021-1136
20 L1021-1137
Ni Chromstar HP40 mg His-tagged protein/mL340.320025 mL1021-1111
100 mL1021-1112
500 mL1021-1113
1 L1021-1114
5 L1021-1115
10 L1021-1116
20 L1021-1117


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