MaXtar PlasmidCap HR is a thiophilic aromatic adsorption chromatography resin. Thiophilic affinity chromatography works by using the interaction between electron donors and acceptors to separate and purify target molecules. This interaction can be strengthened in a high-salt environment and weakened in a low-salt environment. MaXtar PlasmidCap HR can selectively bind to closed-loop supercoiled plasmid DNA but not to open-loop plasmid DNA. Therefore, it can be used to prepare high-quality plasmid DNA.
Unlike PlasmidCap Chromstar HP chromatography resin, MaXtar PlasmidCap HR conjugates the sulfur-containing compound 2-mercaptopyridine to a new highly rigid matrix, which provides better pressure-flow performance for large-scale separation and purification of plasmid DNA, bringing added flexibility in process design and improved productivity. Also, finer particle sizes can provide better resolution.