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Purification Techniques | How to


As a representative of high resolution and good compatibility in downstream separation and purification technology, chromatography technology is widely used in the large-scale preparation of biological macromolecules. According to different separation principles, it can be divided into affinity chromatography, gel filtration chromatography, ion exchange chromatography, hydrophobic chromatography and multi-mode chromatography.


A right resin can meet the project requirements in terms of purity, recovery rate, and other quality aspects; it can also take into account economic dimensions such as high capacity, long life, and excellent amplification performance.


Today, taking affinity chromatography as an example, we will talk about how to choose the "right" chromatography resins.


Quality dimension

Firstly, a correct affinity chromatography resin needs to be tested to determine whether it can achieve the desired process objectives, such as high purity and low residual impurities in quality control, as well as high recovery rate, in order to select the appropriate affinity resin.


Sharing of affinity chromatography case in bispecific antibody project


Purification Techniques | How to "Buy Right and Not Expensive" for Affinity Chromatography?

Figure 1: MaXtar ARPA affinity chromatography map


Purification Techniques | How to "Buy Right and Not Expensive" for Affinity Chromatography?

Table 1: Detection data for MaXtar ARPA affinity chromatography


Economic dimension

Secondly, a "good" affinity chromatography resin material should not only be considered for its excellent process performance, but also for its economic performance of resin material amplification. For example, high binding capacity affinity resin material can reduce the volume of packing material, while also reducing the amount of buffer used, as well as the size of hardware equipment such as chromatography systems and columns, which can bring significant cost advantages. Excellent alkali resistance can increase the service life of resin material. Good mechanical properties can withstand higher flow rate conditions under higher column beds, which is beneficial for amplification production.


purification-techniques-how-to-buy-right-and-not-expensive-for-affinity-chromatography-3.jpg

Figure 2: Comparison of MaXtar ARPA load capacity



Purification Techniques | How to "Buy Right and Not Expensive" for Affinity Chromatography?

Figure 3: Change in cleaning load of MaXtar ARPA CIP


The results in Figure 2 and Figure 3 show that the load capacity of the Bio-Link MaXtar ARPA protein A affinity resin in the monoclonal antibody project is slightly higher than that of domestic competitors. Using the cell culture supernatant containing monoclonal antibodies, the MaXtar ARPA affinity chromatography resin was subjected to more than 100 cycles of CIP test, with each cycle being subjected to 0.5 M NaOH for 15 minutes in situ cleaning. After 100 cycles of test, it still reached 80% of the initial level.


Only buy the "right" and not the "expensive". Bio-Link has launched a full range of affinity chromatography resin products, like industrial chromatography column, has been successfully applied in multiple clinical projects. Please feel free to inquire.

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